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.g.ice-point check)Reference thermocouples Full traceable re-calibration Every 3 yearsCheck against reference thermometer AnnuallyWorking thermometers & Check against reference thermometer AnnuallyWorking thermocouples at ice-point and/or working temperaturerangeBalances Full traceable calibration AnnuallyCalibration weights Full traceable calibration Every 5 yearsCheck weight(s) Check against calibrated weight or Annuallycheck on balance immediately followingtraceable calibrationVolumetric glassware Gravimetric calibration to required AnnuallytoleranceMicroscopes Traceable calibration of stage Initiallymicrometer (where appropriate)Hygrometers Traceable calibration AnnuallyCentrifuges Traceable calibration or check against Annuallyan independent tachometer, asappropriateAppendix E Guidance on equipment validation and verification ofperformanceThis information is provided for guidance purposes and the frequency will be based onthe need, type and previous performance of the equipment.Type of equipment Requirement Suggested frequencyTemperature controlled (a) Establish stability and uniformity of (a) Initially, every 2 years andequipment (incubators, temperature after repair/modificationbaths, fridges, freezers) (b) Monitor temperature (b) Daily/each useSterilising ovens (a) Establish stability and uniformity of (a) Initially, every 2 years andtemperature after repair/modification(b) Monitor temperature (b) Each useAutoclaves (a) Establish characteristics for (a) Initially, every 2 years andloads/cycles after repair/modification(b) Monitor temperature/time (b) Each useJuly 2002 rev02 Page 23 of 26EA - 4/10  Accreditation in Microbiological LaboratoriesType of equipment Requirement Suggested frequencySafety cabinets (a) Establish performance (a) Initially, every year andafter repair/modification(b) Microbiological monitoring (b) Weekly(c) Air flow monitoring (c) Each useLaminar air flow cabinets (a) Establish performance (a) Initially, and afterrepair/modification(b) Check with sterility plates (b) WeeklyTimers Check against national time signal AnnuallyMicroscopes Check alignment Daily/each usepH meters Adjust using at least two buffers of Daily/each usesuitable qualityBalances Check zero, and reading against check Daily/each useweightDe-ionisers and reverse (a) Check conductivity (b) Weeklyosmosis units (b) Check for microbial contamination (b) MonthlyGravimetric diluters (a) Check weight of volume dispensed (a) Daily(b) Check dilution ratio (b) DailyMedia dispensers Check volume dispensed Each adjustment orreplacementPipettors/pipettes Check accuracy and precision of Regularly (to be defined byvolume dispensed taking account of the frequencyand nature of use)Spiral platers (a) Establish performance against (a) Initially and annuallyconventional method(b) Check stylus condition and the start (b) Daily/each useand end points(c) Check volume dispensed (c) MonthlyColony counters Check against number counted AnnuallymanuallyCentrifuges Check speed against a calibrated and Annuallyindependent tachometerAnaerobic jars/incubators Check with anaerobic indicator Each useLaboratory environment Monitor for airborne and surface Weeklymicrobial contamination using, e.g.airsamplers, settle plates, contact platesor swabsJuly 2002 rev02 Page 24 of 26EA - 4/10  Accreditation in Microbiological LaboratoriesAppendix F Guidance on maintenance of equipmentThis information is provided for guidance purposes and the frequency will be based onthe need, type and previous performance of the equipment.Type of equipment Requirement Suggested frequency(a) Incubators Clean and disinfect internal surfaces (a) Monthly(b) Fridges (b) When required (e.g.every(c) Freezers, ovens 3 months)(c) When required (e.g.annually)Water baths Empty, clean, disinfect and refill Monthly, or every 6 months ifbiocide usedCentrifuges (a) Service (a) Annually(b) Clean and disinfect (b) Each useAutoclaves (a) Make visual checks of gasket, (a) Regularly, asclean/drain chamber recommended by(b) Full service manufacturer(c) Safety check of pressure vessel (b) Annually or asrecommended bymanufacturer(c) AnnuallySafety cabinets Full service and mechanical check Annually or as recommendedLaminar flow cabinets by manufacturerMicroscopes Full maintenance service AnnuallypH meters Clean electrode Each useBalances, gravimetric (a) Clean (a) Each usediluters (b) Service (b) AnnuallyStills Clean and de-scale As required (e.g.every 3months)De-ionisers, reverse Replace cartridge/membrane As recommended byosmosis units manufacturerAnaerobic jars Clean/disinfect After each useMedia dispensers, Decontaminate, clean and sterilise as Each usevolumetric equipment, appropriatepipettes, and generalservice equipmentSpiral platers (a) Service (a) Annually(b) Decontaminate, clean and sterilise (b) Each useLaboratory (a) Clean and disinfect working (a) Daily, and during usesurfaces (b) Weekly(b) Clean floors, disinfect sinks and (c) Every 3 monthsbasins(c) Clean and disinfect other surfacesJuly 2002 rev02 Page 25 of 26EA - 4/10  Accreditation in Microbiological LaboratoriesJuly 2002 rev02 Page 26 of 26 [ Pobierz całość w formacie PDF ]